Repeated freezing and thawing is not recommended. Normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance Note: All of our proteins are default shipped with May differ from different purchasing way or location, please kindly consult your local distributors Generally, the shelf life of liquid form is 6 months at -20☌/-80☌. The shelf life is related to many factors, storage state, buffer ingredients, storage temperature Store at -20☌ upon receipt, aliquoting is necessary for mutiple use. Our default final concentration of glycerol is 50%. Recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We We recommend that this vial be briefly centrifuged prior to opening to bring the contents to theīottom. You have specified tag type, please tell us and we will develop the specified tag preferentially. Biol.The tag type will be determined during production process. NMR of Proteins and Nucleic Acids (Wiley, New York, 1986). Biological Macromolecules and Assemblies 2, (eds Jumak, F. CspB binds to single-stranded DNA in gel retardation experiments.
A three-stranded β-sheet, which contains the conserved RNA-binding motif RNP1 as well as a motif similar to RNP2 in two neighbouring antiparallel β-strands 12–14, has basic and aromatic residues at its surface which could serve as a binding site for single-stranded DNA. The structure resembles that of staphylococcal nuclease and the gene-5 single-stranded-DNA-binding protein 9–11. The 67-residue protein consists of an antiparallel five-stranded β-barrel with strands connected by turns and loops. We present here the three-dimensional structure of CspB determined by nuclear magnetic resonance spectroscopy. subtilis (CspB) 8 have sequences that are more than 40 per cent identical to the cold-shock domain. The major cold-shock proteins of Escherichia coli (CS7.4) 4–7 and B. The same domain exists in the sequence of the Xenopus RNA-binding proteins FRG Y 1, and FRG Y 2 (refs 1, 3).
THE cold-shock domain (CSD) is found in many eukaryotic transcriptional factors and is responsible for the specific binding to DNA of a cis-element called the Y-box 1–3.
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